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 l’aide de neuf loci cpSSR, ono a décelé au total 23 haplotypes chloroplastiques et 25 allèles cpSSR parmi 159 sujets provenant de sept populations très dispersées. La diversité génétique totale, HT, était de 0,618, mais la différenciation des haplotypes était faible parmi les populations (GST = 0,121).
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Variation in paternally inherited chloroplast microsatellite (cpSSR) DNA was used to study population genetic structure in red pine (Pinus resinosa Ait.), a species characerized by morphological uniformity, no allozyme variation, and limited RAPD variation. Using nine cpSSR loci, a total of 23 chloroplast haplotypes and 25 cpSSR alleles were found among 159 individuals surveyed in seven widely separated populations. The total genetic diversity, HT, was 0.618, but haplotype differentiation among populations was low (GST = 0.121). All populations were distinguished from each other by their haplotype compositions, and only one haplotype was common among all populations. Based on average squared composite cpSSR length differences (stepwise haplotypes), within-population diversity was relatively high for only one population (D2SH = 0.443). Frequency distributions of pairwise SSR differences among individuals within different populations, as well as branch length differences in neighbour-joining dendrograms, indicated recovery from one or more population bottlenecks, and may be explained by metapopulation dynamics.
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